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Virus‐induced gene silencing of Withania somnifera squalene synthase negatively regulates sterol and defence‐related genes resulting in reduced withanolides and biotic stress tolerance 下载免费PDF全文
65.
Melally Giddegowda Venkatesha Anegunda Shankar Dinesh 《Biocontrol Science and Technology》2011,21(8):929-940
Spalgis epius (Lepidoptera: Lycaenidae) has been recorded as a potential predator of various species of mealybug crop pests worldwide. We describe the mass rearing of S. epius, as no information on this topic is available. Outdoor nylon tent cages of different dimensions were provided to achieve mating and oviposition as S. epius adults did not mate in the laboratory cages. Adults mated only in the tent cage (6×6×10 m) placed over a native tree (9 m height). The presence of a tree canopy inside the cage is essential to achieve courtship and mating. Gravid females of S. epius deposited eggs on the mealybug-infested pumpkins inside the different sized nylon cages with or without a bush/tree. Spalgis epius eggs were maintained on mealybug-infested pumpkins in the laboratory and developmental stages of the predator were reared. Adults fed on various diets laid significantly higher number of eggs than those of starved individuals. Spalgis epius with a life cycle completed in 21.2 days and 55.7 larvae, could be reared on a single mealybug-infested pumpkin. 相似文献
66.
Prasad Dinesh Shankaracharya Vidyarthi Ambarish Sharan 《World journal of microbiology & biotechnology》2011,27(9):2227-2230
A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric
assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel
with the conventional culture method. The sensitivity and specificity of colorimetric assay was 89.15 and 99.04% respectively
with reference to conventional culture method. The total time required to detect the Salmonella spp. present in food samples by the developed method is less than 8 h, including 6 h incubation. It was observed that the
colorimetric assay was 10 times more sensitive than gel-based detection with the same concentration of DNA used for analysis. 相似文献
67.
Development of efficient strategies and automation represent important milestones of progress in rapid structure determination
efforts in proteomics research. In this context, we present here an efficient algorithm named as AUTOBA (Automatic Backbone
Assignment) designed to automate the assignment protocol based on HN(C)N suite of experiments. Depending upon the spectral
dispersion, the user can record 2D or 3D versions of the experiments for assignment. The algorithm uses as inputs: (i) protein
primary sequence and (ii) peak-lists from user defined HN(C)N suite of experiments. In the end, one gets HN, 15N, Cα and C′ assignments (in common BMRB format) for the individual residues along the polypeptide chain. The success of the algorithm
has been demonstrated, not only with experimental spectra recorded on two small globular proteins: ubiquitin (76 aa) and M-crystallin
(85 aa), but also with simulated spectra of 27 other proteins using assignment data from the BMRB. 相似文献
68.
Zaware P Shah SR Pingali H Makadia P Thube B Pola S Patel D Priyadarshini P Suthar D Shah M Jamili J Sairam KV Giri S Patel L Patel H Sudani H Patel H Jain M Patel P Bahekar R 《Bioorganic & medicinal chemistry letters》2011,21(2):628-632
A novel series of oxime containing benzyl-1,3-dioxane-r-2-carboxylic acid derivatives (6a-k) were designed as selective PPARα agonists, through bioisosteric modification in the lipophilic tail region of PPARα/γ dual agonist. Some of the test compounds (6a, 6b, 6c and 6f) showed high selectivity towards PPARα over PPARγ in vitro. Further, highly potent and selective PPARα agonist 6c exhibited significant antihyperglycemic and antihyperlipidemic activity in vivo, along with its improved pharmacokinetic profile. Favorable in-silico interaction of 6c with PPARα binding pocket correlate its in vitro selectivity profile toward PPARα over PPARγ. Together, these results confirm discovery of novel series of oxime based selective PPARα agonists for the safe and effective treatment of various metabolic disorders. 相似文献
69.
Garcia E Andrews C Hua J Kim HL Sukumaran DK Szyperski T Odunsi K 《Journal of proteome research》2011,10(4):1765-1771
We show that (1)H NMR based metabonomicsof serum allows the diagnosis of early stage I/II epithelial ovarian cancer (EOC) required for successful treatment. Because patient specimens are highly precious, we conducted an exploratory study using a microflow probe requiring only 20 μL of serum. By use of logistic regression on principal components (PCs) of the NMR profiles, we built a 4-variable model for early stage EOC prediction (training set: 69 EOC specimens, 84 healthy controls; test set: 40 EOC, 44 controls) with operating characteristics estimated for the test set at 80% specificity [95% confidence interval (CI): 65-90%], 63% sensitivity (95% CI: 46-77%), and an area under the Receiver Operator Characteristic Curve (AUC) of 0.796. Independent validation (50 EOC, 50 controls) of the model yielded 95% specificity (95% CI: 86-99.5%), 68% sensitivity (95% CI: 53-80%) and an AUC of 0.949. A test on cancer type specificity showed that women diseased with renal cell carcinoma were not incorrectly diagnosed with EOC, indicating that metabonomics bears significant potential for cancer type-specific diagnosis. Our model can potentially be applied for women at high risk for EOC, and our study promises to contribute to developing a screening protocol for the general population. 相似文献
70.
Bernstein PA Wecker D Krishnamurthy A Manocha D Gardner J Kolker N Reschke C Stombaugh J Vagata P Stewart E Welch D Kolker E 《Omics : a journal of integrative biology》2011,15(4):203-207
This article is a summary of the technology issues and challenges of data-intensive science and cloud computing as discussed in the Data-Intensive Science (DIS) workshop in Seattle, September 19-20, 2010. 相似文献